![cell stereology trypan blue cell stereology trypan blue](http://www.nexcelom.com/images/isolated-human-T-cells-stained-with-trypan-blue.gif)
They might be able to help you troubleshoot and suggest the right kit. I would suggest that you call technical support and explain your problem. And try to contact the Invitrogen sales Rep from your area.the Rep might be able to give you a sample/trial kit to test the staining on your cells.
![cell stereology trypan blue cell stereology trypan blue](https://slidetodoc.com/presentation_image/9842388054163bbd8a1bc0773a43212e/image-13.jpg)
The dye in bacterial kit is SYTO® 9 dye propidium iodide. I have used the live/dead staining from invitrogen for bacteria, which worked well. Unfortunately, I don't have any experience working with mammalian cells. Invitrogen has 4 different kits which use 4 different dyes, could you please specify which one worked best for you. I have not had much luck using this particular Promega assay kit for cell viability. The LIVE/DEAD® Cell Vitality Assay Kit *C12-resazurin/SYTOX® Green* *1000 assay has the same dye (REsazurin) as in the Promega Cell Titer Blue kit. They have a couple of those for mammalian cells. I looked up the invitrogen website for the live/ dead stain. Have you been able to get consistent results using the invitrogen live/ dead stain? I wonder if there is a way to stain all the cells including live cells with a different color to help better visualisation of all cells That would. Trypan blue makes the nuclei of damaged cells dark blue. I plate 5000 cells/ well in a 96-well plate. Cunterstaining Trypan blue - posted in Cell Biology: I am meaguring of cytotoxicity using cell culture in 24 well plate and staining dead cells directly on the plate with Trypan blue. I have been experiencing problems and see variable results each time. Have you tried using Promega's Cell Titer Glo Cell Viability assay kit. Below is the website for the stain.check it out.maybe it is applicable for what you are trying to do. There is a live/dead stain (fluorescent-based) available from Invitrogen. I tried Eosin staining and it seems to me that ethanol in Eosin stain is washing away the Trypan Blue color. I wonder if there is a way to stain all the cells including live cells with a different color to help better visualisation of all cells? That would especially help for better photographs and image analysis. I am meaguring of cytotoxicity using cell culture in 24 well plate and staining dead cells directly on the plate with Trypan blue.